The Chilean football federation asked FIFA to investigate its claim that Ecuador player Byron Castillo is actually Colombian and not entitled to have played in qualifying games.
„FIFA can confirm that it has received a formal complaint from the Chilean FA in relation to this matter,“ football’s governing body said in a statement.
FIFA gave no timetable for a possible disciplinary case ahead of the World Cup kicking off Nov. 21.
Ecuador placed fourth in the 10-nation South American qualifying group to advance directly to Qatar.
Chile placed seventh, seven points behind Ecuador, but argue they could advance if games involving Castillo were forfeited.
The FIFA rules in cases of ineligible players require results to be overturned as a 3-0 loss.
Bolivia lost an appeal at the Court of Arbitration for Sport which said FIFA was right to investigate even when protests were filed weeks after the games were played.
FIFA wrote stricter rules for the 2022 World Cup requiring all players in qualifying games to produce a „valid permanent international passport“ for inspection by match officials.